Delivering on the Promise of Purity

Tris HCL

TRIS HCl (molecular formula NH2C(CH2OH)3 · HCl), or Tris(hydroxymethyl)aminomethane hydrochloride, is a versatile buffering agent widely utilized in the pharmaceutical industry. This chemical compound plays a crucial role in maintaining a stable pH environment, which is vital for various biochemical and pharmaceutical processes. 

Properties of TRIS HCl:

TRIS HCl (molecular weight 157.60g/mol) is a white crystalline powder that is highly soluble in water    (667 mg/ml), making it easily incorporated into various pharmaceutical formulations. Its chemical structure consists of a trihydroxymethylaminomethane molecule combined with a hydrochloride group. The pKa value of TRIS HCl is approximately 8.1 at 25°C, which makes it an effective buffer within the physiological pH range.

Benefits of TRIS HCl in Pharmaceutical Manufacturing:

  1. pH Regulation: TRIS HCl is known for its exceptional buffering capacity, especially within the pH range of 7.2 to 9.0. This makes it ideal for applications where a stable pH environment is crucial, such as the manufacturing of pharmaceuticals. Maintaining the desired pH is essential for the stability and efficacy of drugs, as well as for the reproducibility of various biochemical processes.
  1. Biocompatibility: TRIS HCl is considered biocompatible, meaning it is generally well-tolerated by living organisms. This characteristic is particularly important in pharmaceutical formulations intended for human use. The compatibility of TRIS HCl with biological systems makes it a preferred choice for buffering solutions in drug development.
  1. Versatility: TRIS HCl is versatile and can be used in various pharmaceutical applications, including protein and enzyme purification, cell culture, and nucleic acid isolation. Its compatibility with a wide range of substances makes it a valuable buffering component for pharmaceutical manufacturers working with diverse formulations.
  1. Stability: The stability of TRIS HCl, both in solid form and in solution, contributes to its reliability as a buffering agent. Pharmaceutical products often undergo extended storage periods, and the stability of the buffering system becomes crucial in maintaining the integrity of the formulation over time.
  2. Minimal Interference: TRIS HCl exhibits minimal interference with biochemical and enzymatic reactions. This property is vital in ensuring that the buffering component does not negatively impact the performance of the pharmaceutical product. The low reactivity of TRIS HCl enhances its applicability in various drug development processes.
Blue barrel of Pfanstiehl high purity GMP excipient grade Tris HCL powder

High Purity – Low Endotoxin – Low Metals – Parenteral Grade

TRIS HCl is a valuable buffering component in the pharmaceutical industry due to its excellent pH-regulating properties, biocompatibility, versatility, stability, and minimal interference with biochemical processes. 

Highest Quality for your Highest Risk Applications

Pfanstiehl’s TRIS HCL (CAS 6138-23-4) was developed specifically for biopharmaceutical formulation and commercial manufacturing and is produced under full ICH-Q7 cGMP conditions in the United States to the highest quality and purity standards.  

Because of these stringent manufacturing specifications and quality systems, you can be assured that Pfanstiehl’s TRIS HCL will be of the highest quality & consistency from batch to batch and the safest available for use in your manufacturing or research application. 

Have questions or interested in a development sample?  

Please Click on the Contact Us below and one of our world-class Technical Support Representatives will contact you.

Product DescriptionProduct CodeCAS Number
Tris HCl High Purity, Low Endotoxin, Low metals T-1541185-53-1

Available Package Sizes: 1kg, 5kg, 10kg, 25kg

Development Samples: ~100g – Available upon request