Delivering on the Promise of Purity

Tris Base USP EP BP ChP

Tris Base (molecular formula NH2C(CH2OH)3), or tris(hydroxymethyl)aminomethane, is a commonly used buffer in biologics production and various biochemical and molecular biology applications. It is a zwitterionic amine, meaning it has both acidic and basic functional groups in its structure, making it an effective buffer over a wide pH range.

Here are some benefits of using Tris Base (molecular weight 121.14g/mol) in buffering applications in biologics production:

  1. pH Stability: Tris Base is known for its ability to maintain a stable pH over a broad range, typically between pH 7 and 9. This is crucial in biologics production processes where maintaining a specific pH is essential for the stability and functionality of proteins.
  1. Biocompatibility: Tris is relatively biologically inert, which is important when working with sensitive biological molecules. It minimizes the potential for interference with biochemical reactions or interactions.
  1. Water Solubility: Tris Base is highly soluble in water, making it easy to prepare stock solutions for use in various applications. This solubility is essential in biologics production where precise and reproducible conditions are necessary.
  1. Low UV Absorbance: Tris exhibits low ultraviolet (UV) absorbance, which is important when working with biomolecules that are sensitive to UV light. Low absorbance helps prevent interference with spectrophotometric measurements.
  1. Buffering Capacity: Tris has a good buffering capacity, allowing it to resist changes in pH when small amounts of acid or base are added. This is critical in maintaining a stable environment for biologics during various stages of production.
  1. Non-Toxicity: Tris is generally considered non-toxic at typical working concentrations, which is crucial for applications involving cells or proteins where cytotoxicity could be a concern.
  2. Versatility: Tris Base is versatile and can be used in a variety of applications, including electrophoresis, protein purification, enzyme assays, and other molecular biology techniques.
Blue barrel of Pfanstiehl high purity excipient grade Tris Base powder

High Purity – Low Endotoxin – Low Metals – Parenteral Grade

Highest Quality for your Highest Risk Applications

Pfanstiehl Brand High Purity, Low endotoxin, Low metals, Tris Base was specifically developed for use in Biopharmaceutical manufacturing and is manufactured in the USA under ICH-Q7 cGMP conditions and in compliance with USP/EP/BP/ and ChP Pharmacopeia.

Because of these stringent manufacturing specifications and quality systems, you can be assured that Pfanstiehlโ€™s Tris Base will be of the highest consistency from batch to batch and the safest available for use in your drug product formulation or research application. 

Have questions or interested in a development sample?  

Please Click on the Contact Us below and one of our world-class Technical Support Representatives will contact you.

Product DescriptionProduct CodeCAS Number
Tris Base High Purity, Low Endotoxin, Low metals USP, EP, BP, ChPT-15377-86-1

Available Package Sizes: 1kg, 5kg, 10kg, 25kg

Development Samples: ~100g – Available upon request