L-Arginine HCL USP EP JP ChP

Pfanstiehl Multi-Compendial L-Arginine HCl serves several purposes in Biologics Production:

Cell Culture Media

L-Arginine HCl is often included in cell culture media to support the growth and viability of cells. It provides a nitrogen source for cell metabolism and protein synthesis, which is crucial for the production of biologics such as monoclonal antibodies, recombinant proteins, and viral vectors in cell culture systems.

Stabilizer

L-Arginine HCl can act as a stabilizer in biologics formulations, helping to maintain the stability and integrity of proteins and other biologics molecules during storage and transportation. It can protect against denaturation and aggregation, which are common challenges in the formulation of biologics.

Facilitates Protein Refolding

During the production of recombinant proteins, L-Arginine HCl can be used to facilitate protein refolding. Protein refolding is a critical step in the production of biologics, particularly when producing proteins in bacterial expression systems like E. coli, where proteins often fold incorrectly and form insoluble aggregates known as inclusion bodies. L-Arginine HCl can help solubilize and refold these proteins into their biologically active conformations.