L-Methionine USP EP BP JP ChP

Pfanstiehl Multi-Compendial L-Methionine benefits in Cell Culture:

Protein synthesis

L-Methionine is an essential building block for proteins. It is the initiator amino acid in the synthesis of polypeptide chains, meaning that it is the first amino acid incorporated into a growing protein chain during translation.

Methylation reactions

L-Methionine is a precursor for S-adenosylmethionine (SAM), which is a universal methyl donor involved in a wide range of methylation reactions within cells. Methylation reactions are important for regulating gene expression, protein function, and various metabolic pathways.

Antioxidant activity

L-Methionine possesses antioxidant properties, helping to protect cells from oxidative stress. Oxidative stress occurs when there is an imbalance between the production of reactive oxygen species (ROS) and the body’s ability to detoxify them. ROS can cause damage to cellular components such as DNA, proteins, and lipids. L-Methionine acts as a scavenger of free radicals and helps prevent oxidative damage to cells.

Pfanstiehl Multi-Compendial L-Methionine has antioxidant properties in Biologics Formulations due to:

Reactive oxygen species (ROS) scavenging

L-Methionine can directly react with and neutralize reactive oxygen species, such as superoxide anion (O2-) and hydroxyl radicals (OH•), thereby preventing oxidative damage to the monoclonal antibodies.

Metal chelation

L-Methionine can chelate transition metal ions, such as iron and copper, which are known to catalyze the generation of reactive oxygen species through Fenton and Haber-Weiss reactions. By chelating these metal ions, L-Methionine inhibits their ability to generate ROS and reduces oxidative stress.

Regeneration of other antioxidants

L-Methionine can regenerate other antioxidants, such as glutathione and vitamin E, which are involved in cellular antioxidant defense mechanisms. By regenerating these antioxidants, L-Methionine enhances the overall antioxidant capacity of the monoclonal antibody formulation.